Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Detecting the effects of pharmacological agents on SIRT1. Screening inhibitors or activators of SIRT1.ģ. The assay may be used to follow the purification of Sirtuins or may be used to detect the presence of Sirtuins in cell lysates.Ģ. The assay shows good linearity of sample response. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT1 activity with the SIRT1 Activity Assay Kit if the appropriate antibody directed against SIRT1 (Anti-SIRT1 antibody ( ab7343)) is used for immunoprecipitation.Ībcam’s SIRT1 Activity Assay Kit (Fluorometric) has been shown to detect the activity of Sirtuins, at least SIRT1 in Human or animal cell lysates or in column fractions.
Primarily, the SIRT1 Activity Assay Kit is designed for the rapid and sensitive evaluation of SIRT1 inhibitors or activators using crude SIRT1 fraction or purified SIRT1. SIRT1 Activity Assay Kit (Fluorometric) (ab156065) detects SIRT1 activity in lysates.